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Un trabajo citado en blazei.org sobre las diferentes morfologías y composiciones químicas del Agaricus blazei dependiendo de la cepa micelial, modo de cultivo y localización del mismo
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Resumen de literatura científica de alto índice de impacto sobre el Agaricus blazei Murill o champiñón del Sol
Acciones de Documento
Genetic variation in cultivated strains of Agaricus blazei
2004 - Variación genética en las cepas cultivadas de Agaricus blazei.
Palabras clave:
distintos tipos
| Publicación | Mycoscience |
| Editor | Springer Japan |
| ISSN | 1340-3540 (Print) 1618-2545 (Online) |
| Fascículo/ejemplar/número | Volume 44, Number 6 / diciembre de 2003 |
| Categoría | FULL PAPER |
| DOI | 10.1007/s10267-003-0136-x |
| Páginas | 431-436 |
| Subject Collection | Biomedicina y ciencias biológicas |
| Fecha de SpringerLink | jueves, 19 de febrero de 2004 |
Masaki Fukuda1 
, Shotaro Ohno2 and Masako Kato2
| (1) | Graduate School of Agriculture, Shinshu University, Minamiminowa, Nagano, 399-4598, Japan |
| (2) | Faculty of Agriculture, Shinshu University, Nagano, Japan |
Received: 28 August 2003 Accepted: 07 August 2003
Abstract Genetic differences among Agaricus blazei
strains were investigated using somatic incompatibility testing,
isozyme analysis, restriction fragment length polymorphism (RFLP)
analysis of mitochondrial DNA (mtDNA), and random amplified polymorphic
DNA (RAPD) analysis. Eight strains, one cultivated strain from Brazil
and seven from Japan, were used in this study. Somatic incompatibility
interactions were observed between the Brazilian cultivated strain and
the Japanese strains. The Brazilian cultivated strain had its own
distinct patterns of esterase isozyme and mtDNA RFLP, but all seven
Japanese cultivated strains showed identical patterns. When the RAPD
patterns, obtained using eight primers, were compared the eight strains
had their own distinct RAPD profiles. Distance values were calculated
between all pairs of the strains based on presence or absence of
individual RAPD bands, and a dendrogram was constructed by unweighted
pair-group method with arithmetic clustering (UPGMA) analysis. Seven
Japanese cultivated strains were grouped to each other, and this group
was finally linked to the Brazilian cultivated strain. Based on these
results, the degree of genetic variation among the A. blazei strains used is discussed.
