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Acciones de Documento
Measurement of a genotoxic hydrazine, agaritine, and its derivatives by HPLC with fluorescence derivatization in the agaricus mushroom and its products
2006 - Medición de un genotóxico hidracina, agaritine, y sus derivados por HPLC con derivatización de fluorescencia en las setas Agaricus y sus productos
- Chem Pharm Bull (Tokyo). 2006 Jun;54(6):922-4.
Measurement of a genotoxic hydrazine, agaritine, and its derivatives by HPLC with fluorescence derivatization in the agaricus mushroom and its products.
Nagaokaa MH, Nagaoka H, Kondo K, Akiyama H, Maitani T.
National Institute of Health Sciences, Japan.
Agaricus blazei MURRILL mushroom products are sold as so-called health foods in Japan. However, a part of Agaricus is known to contain hydrazines. A sensitive and specific method for analyzing a genotoxic hydrazine, agaritine, and its derivatives was developed to assess the safety of Agaricus products. beta-N-(gamma-L(+)-Glutamyl)-4-(hydroxymethyl)phenylhydrazine (agaritine, AGT), 4-hydrazinylbenzylalcohol (HMPH), 4-hydrazinylbenzoic acid (CPH), 4-methylphenylhydrazine (MPH) and phenylhydrazine (PH) were converted to their correspond-ing fluorescent derivatives with 3,4-dihydro-6,7 dimethoxy-4-methyl-3-oxoquinoxaline-2-carbonyl chloride (DMEQ-COCl) as the fluorescence derivatization reagent. The detection limits (S/N=3) for CPH, AGT, PH and MPH were 422, 45.3, 16.5 and 138 fmol, respectively, in a 20 mul injection volume. Recoveries, achieved by adding known AGT amounts to the Agaricus sample and Agaricus products, ranged from 92.8 to 102%. By using this method which does not require partial purification of the Agaricus sample, the amounts of AGT in several types of foods were found to be 112-1836 mug/g dry weight.
Dos nuevos trabajos científicos citados en Pubmed sobre las actividades antioxidantes, antimutagénicas y anti-hepatoma del Agaricus blazei